Development of Highly Polymorphic Pentanucleotide Tandem Repeat Loci with Low Stutter
نویسنده
چکیده
INTRODUCTION All eukaryotic genomes contain regions of simple repetitive DNA, called short tandem repeats (STR*) or microsatellites, which consist of tandem repeats of a small number of bases (1-3). The number of repeats at a STR locus can be highly variable among individuals, resulting in length polymorphisms that can be detected by relatively simple PCR-based assays. Thousands of these highly informative STR loci have been identified in the human genome. Consequently, STRs provide an abundant class of polymorphic markers that have gained popularity for use in genetic linkage analysis, mapping, identity testing and many other applications (3-6).
منابع مشابه
Genetic Variation of Informative Short Tandem Repeat (STR) Loci in an Iranian Population
In the present study, genotyping of six short tandem repeat (STR) loci including CSF1PO, D16S539, F13A01, F13B, LPL and HPRTB was performed on genomic DNA from 127 unrelated individuals from the Iranian province of Isfahan. The results indicated that the allele and genotype distributions were in accordance with Hardy-Weinberg expectations. The observed heterozygosity (Ho), expected heterozygosi...
متن کاملGenotyping of Five Polymorphic STR Loci in Iranian Province of Isfahan
Genotyping for five short tandem repeat (STR) loci HUMvWA, HUMFES, HUMTPO, HUMTH01 and D3S1359 was done in 220 unrelated individuals from the population of Isfahan province of IR Iran. The loci were genotyped using the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and silver staining. The data demonstrated that the STR markers were all found informative i...
متن کاملDiversity of Variable Number Tandem Repeat Loci in Shigella Species Isolated From Pediatric Patients
Multilocus variable number tandem repeat (VNTR) analysis (MLVA) is a new typing method with several advantages compared to other methods. Dissemination of Shigella is highly significant in developing countries. Whilst Shigella is becoming increasingly important as an etiologic agent of pediatric shigellosis in Iran, little is known about the genetic diversity of the local strains. Therefore, th...
متن کاملVNTR9 and VNTR10, two newly-found variable-number tandem repeat loci useful in MLVA genotyping of Bordetella pertussis
Background & Aims: Bordetella pertussis, the causative agent of whooping cough, continues to infect human hosts even in those populations where infants and children are routinely vaccinated. Causes of pertussis epidemiology are not fully identified unless strains of the pathogen are characterized by molecular means. Golbally, Multi Locus Variable Number of Tandem Repeats analysis (MLVA) has pro...
متن کاملSequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA.
The PCR amplification of tetranucleotide short tandem repeat (STR) loci typically produces a minor product band 4 bp shorter than the corresponding main allele band; this is referred to as the stutter band. Sequence analysis of the main and stutter bands for two sample alleles of the STR locus vWA reveals that the stutter band lacks one repeat unit relative to the main allele. Sequencing result...
متن کامل